Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance

Tohru Fujiwara; Yoko Okitsu; Yuna Katsuoka; Noriko Fukuhara; Yasushi Onishi; Kenichi Ishizawa; Hideo Harigae

doi:10.1016/j.fob.2013.10.004

Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance

FEBS Open Bio. 2013 Oct 11:3:428-32. doi: 10.1016/j.fob.2013.10.004. eCollection 2013.

Authors

Tohru Fujiwara¹, Yoko Okitsu, Yuna Katsuoka, Noriko Fukuhara, Yasushi Onishi, Kenichi Ishizawa, Hideo Harigae

Affiliation

¹ Hematology and Rheumatology, Tohoku University Graduate School of Medicine, Sendai, Japan ; Molecular Hematology/Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan.

Abstract

ETO2 is a component of a protein complex containing master regulators of hematopoiesis, including GATA-1 and SCL/TAL1, and also has RNA binding properties. Although ETO2 has been reported to repress GATA-1 target genes through histone deacetylation of the target gene loci in erythroid cells, little is known about the contribution of ETO2 to microRNA (miRNA) regulation. Here, we conducted miRNA profiling in ETO2-overexpressing and ETO2-silenced K562 cells. The analysis suggests that ETO2 positively regulates the abundance of mature miRNAs, including miR-21, miR-29b and let-7e. Our data suggest a novel mode of ETO2-mediated target gene repression via effects on miRNA expression.

Keywords: CBF1, core-binding factor 1; ETO2; ETO2 (CBFA2T3), core-binding factor, runt domain, alpha subunit 2, translocated to, 3; Erythropoiesis; IL-3, interleukin 3; IMDM, Iscove’s Modified Dulbecco’s Media; LMO2, LIM domain only 2; RPMI, Roswell Park Memorial Institute; SCF, stem cell factor; cDNA, complementary DNA; miRNA; siRNA, small interfering RNA.