Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay

Qiuhua Chen; Lu Yuan; Jun Wan; Yanlin Chen; Chaoyang Du

doi:10.1016/j.jviromet.2013.11.006

Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay

J Virol Methods. 2014 Mar:197:29-33. doi: 10.1016/j.jviromet.2013.11.006. Epub 2013 Nov 21.

Authors

Qiuhua Chen¹, Lu Yuan², Jun Wan³, Yanlin Chen¹, Chaoyang Du⁴

Affiliations

¹ The 171 hospital of People's Liberation Army, 332000 Jiang xi, China.
² People's Liberation Army, No 452 Hospital of Chengdu, 610021 Sichuan, China.
³ Chinese PLA 458th Hospital, 510602 Guangdong, China.
⁴ The 171 hospital of People's Liberation Army, 332000 Jiang xi, China. Electronic address: [email protected].

PMID: 24269797
DOI: 10.1016/j.jviromet.2013.11.006

Abstract

Hepatitis E virus (HEV) infection is a common communicable disease in the third world countries. The early diagnosis of HEV is important for the prognosis and prevention of the spread of the disease. Conventional methods for HEV detection need expensive equipment and cannot meet the needs of point of care (POC) use. In the present study, a gold nanoparticle (AuNP) based RT-LAMP assay was developed for colorimetric detection of HEV. As low as 10(1) copies of HEV RNA were detected visually. With reduced handling and less equipment requirement, this assay provided a simple and rapid alternative to other available methods.

Keywords: Colorimetric detection; Hepatitis E virus; Loop mediated isothermal amplification.

Publication types

Evaluation Study

MeSH terms

Colorimetry / methods*
Gold
Hepatitis E / diagnosis*
Hepatitis E / virology
Hepatitis E virus / isolation & purification*
Humans
Molecular Diagnostic Techniques / methods*
Nanoparticles
Nucleic Acid Amplification Techniques / methods*
Reverse Transcription

Substances

Gold