In this study, the full-length cDNA of the UDP-glucose pyrophosphorylase gene was isolated from jute by homologous cloning (primers were designed according to the sequence of UGPase gene of other plants) and modified RACE techniques; the cloned gene was designated CcUGPase. Using bioinformatic analysis, the gene was identified as a member of the UGPase gene family. Real-time PCR analysis revealed differential spatial and temporal expression of the CcUGPase gene, with the highest expression levels at 40 and 120d. PCR and Southern hybridization results indicate that the gene was integrated into the jute genome. Overexpression of CcUGPase gene in jute revealed increased height and cellulose content compared with control lines, although the lignin content remained unchanged. The results indicate that the jute UGPase gene participates in cellulose biosynthesis. These data provide an important basis for the application of the CcUGPase gene in the improvement of jute fiber quality.
Keywords: ADP-glucose pyrophosphorylase; AGPase; CcUGPase; Cellulose; Jute; PPi; Quality; Southern hybridization; UDP; UDP-glucose pyrophosphorylase; UDPG; UGPase; UTP; d; days; pyrophosphate; uridine diphosphate; uridine diphosphate glucose; uridine triphosphate.
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