Abstract
Fifty-seven carbapenem-resistant Klebsiella pneumoniae isolates belonging to ST11 (50 isolates), ST423 (5 isolates), and two other sequence types were studied. All were positive for blaKPC-2, blaTEM-1, and blaCTX-M-14. SDS-PAGE analysis of six representative isolates demonstrated varied porin expression. Nevertheless, when blaKPC-2 was deleted, carbapenem resistance was markedly reduced. Additionally, SHV-12, DHA-1, and/or VIM-1 appeared to contribute to accessory carbapenemase activity. In contrast, OmpK35 and/or OmpK36 deficiency seemed to serve only as a minor cooperative factor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Carbapenems / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation, Bacterial*
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Humans
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / enzymology
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Molecular Sequence Data
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Plasmids
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Porins / genetics*
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Porins / metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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OmpK35 porin, Klebsiella pneumoniae
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OmpK36 protein, Klebsiella pneumoniae
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Porins
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VIM-1 metallo-beta-lactamase
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beta-lactamase SHV-12
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beta-Lactamases
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beta-lactamase KPC-2, Klebsiella pneumoniae
Associated data
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GENBANK/JX276743
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GENBANK/JX291113
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GENBANK/JX291114
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GENBANK/JX310550
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GENBANK/JX310551
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GENBANK/JX310552
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GENBANK/JX310553
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GENBANK/JX310554
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GENBANK/JX310555
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GENBANK/JX310556
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GENBANK/JX310557