Monitoring acute phase proteins in retrovirus infected cats undergoing feline interferon-ω therapy

J Small Anim Pract. 2014 Jan;55(1):39-45. doi: 10.1111/jsap.12160. Epub 2013 Nov 27.

Abstract

Objectives: Recombinant feline interferon-ω therapy is an immunomodulator currently used in the treatment of different retroviral diseases including feline immune deficiency virus and feline leukaemia virus. Although its mechanism of action remains unclear, this drug appears to potentiate the innate response. Acute phase proteins are one of the key components of innate immunity and studies describing their use as a monitoring tool for the immune system in animals undergoing interferon-ω therapy are lacking. This study aimed to determine whether interferon-ω therapy influences acute phase protein concentrations namely serum amyloid-A, α-1-glycoprotein and C-reactive protein.

Methods: A single-arm study was performed using 16 cats, living in an animal shelter, naturally infected with retroviruses and subjected to the interferon-ω therapy licensed protocol. Samples were collected before (D0), during (D10 and D30) and after therapy (D65). Serum amyloid-A and C-reactive protein were measured by specific enzyme-linked immunosorbent assay kits and α-1-glycoprotein by single radial immunodiffusion.

Results: All the acute phase proteins significantly increased in cats undergoing interferon-ω therapy (D0/D65: P<0·05) CLINICAL SIGNIFICANCE: Acute phase proteins appear to be reasonable predictors of innate-immune stimulation and may be useful in the individual monitoring of naturally retroviral infected cats undergoing interferon-ω therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • C-Reactive Protein / analysis*
  • Cat Diseases / blood
  • Cat Diseases / drug therapy*
  • Cat Diseases / virology
  • Cats
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Female
  • Immunodeficiency Virus, Feline
  • Interferon Type I / therapeutic use*
  • Lentivirus Infections / blood
  • Lentivirus Infections / drug therapy
  • Lentivirus Infections / veterinary
  • Leukemia Virus, Feline
  • Leukemia, Feline / blood
  • Leukemia, Feline / drug therapy
  • Male
  • Orosomucoid / analysis*
  • Retroviridae Infections / blood
  • Retroviridae Infections / drug therapy
  • Retroviridae Infections / veterinary*
  • Serum Amyloid A Protein / analysis*

Substances

  • Interferon Type I
  • Orosomucoid
  • Serum Amyloid A Protein
  • interferon omega 1
  • C-Reactive Protein