Abstract
Crowd impact: Molecular crowding effects of bovine serum albumin and poly(ethylene glycol) on the Escherichia coli RecA-mediated strand exchange reaction are quantified by using a single-molecule outgoing strand experiment and magnetic pull-down and ATPase assays. The alterations of the biochemical parameters of this complex enzymatic reaction in such crowded environments are discussed.
Keywords:
DNA; dehydration; filaments; molecular crowding; strand exchange.
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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DNA, Single-Stranded / genetics
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DNA, Single-Stranded / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Nucleoproteins / chemistry*
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Nucleoproteins / genetics
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Nucleoproteins / metabolism
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Polyethylene Glycols / chemistry*
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Rec A Recombinases / chemistry*
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Rec A Recombinases / genetics
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Rec A Recombinases / metabolism
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Serum Albumin, Bovine / chemistry
Substances
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DNA, Single-Stranded
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Escherichia coli Proteins
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Nucleoproteins
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Serum Albumin, Bovine
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Polyethylene Glycols
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Rec A Recombinases