Live cell visualization of Golgi membrane dynamics by super-resolution confocal live imaging microscopy

Kazuo Kurokawa; Midori Ishii; Yasuyuki Suda; Akira Ichihara; Akihiko Nakano

doi:10.1016/B978-0-12-417164-0.00014-8

Live cell visualization of Golgi membrane dynamics by super-resolution confocal live imaging microscopy

Methods Cell Biol. 2013:118:235-42. doi: 10.1016/B978-0-12-417164-0.00014-8.

Authors

Kazuo Kurokawa¹, Midori Ishii, Yasuyuki Suda, Akira Ichihara, Akihiko Nakano

Affiliation

¹ Live Cell Molecular Imaging Research Team, Extreme Photonics Research Group, RIKEN Center for Advanced Photonics, Wako, Saitama, Japan.

PMID: 24295310
DOI: 10.1016/B978-0-12-417164-0.00014-8

Abstract

Super-resolution confocal live imaging microscopy we developed provides cutting-edge high-speed live cell imaging at high space resolution. With this technology we are now able to observe details of membrane traffic events, including behaviors of small vesicles, cisternal maturation of the Golgi apparatus, and membrane segregation within a compartment.

Keywords: Cisternal maturation; Live imaging; Multicolor visualization; Spinning disk confocal; Super-resolution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Golgi Apparatus / metabolism*
Microscopy, Confocal
Microscopy, Fluorescence
Protein Transport
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae / ultrastructure*
Saccharomyces cerevisiae Proteins / metabolism
Single-Cell Analysis

Substances

Saccharomyces cerevisiae Proteins