Generating rats with conditional alleles using CRISPR/Cas9

Cell Res. 2014 Jan;24(1):122-5. doi: 10.1038/cr.2013.157. Epub 2013 Dec 3.

Authors

Yuanwu Ma¹, Xu Zhang¹, Bin Shen², Yingdong Lu¹, Wei Chen¹, Jing Ma¹, Lin Bai¹, Xingxu Huang², Lianfeng Zhang¹

Affiliations

¹ Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Beijing 100021, China.
² MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing Biomedical Research Institute, National Resource Center for Mutant Mice, Nanjing, Jiang Su 210061, China.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Animals
CRISPR-Associated Proteins / genetics*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
DNA (Cytosine-5-)-Methyltransferase 1
DNA (Cytosine-5-)-Methyltransferases / genetics*
DNA Methyltransferase 3A
DNA Methyltransferase 3B
Gene Knockout Techniques
Rats
Rats, Transgenic

Substances

CRISPR-Associated Proteins
DNMT3A protein, human
DNA (Cytosine-5-)-Methyltransferase 1
DNA (Cytosine-5-)-Methyltransferases
DNA Methyltransferase 3A