3'-Hydroxymethyl 2'-deoxynucleoside 5'-triphosphates are inhibitors highly specific for reverse transcriptase

T V Kutateladze; A M Kritzyn; V L Florentjev; V M Kavsan; Z G Chidgeavadze; R S Beabealashvilli

doi:10.1016/0014-5793(86)81489-2

3'-Hydroxymethyl 2'-deoxynucleoside 5'-triphosphates are inhibitors highly specific for reverse transcriptase

FEBS Lett. 1986 Oct 27;207(2):205-12. doi: 10.1016/0014-5793(86)81489-2.

Authors

T V Kutateladze, A M Kritzyn, V L Florentjev, V M Kavsan, Z G Chidgeavadze, R S Beabealashvilli

PMID: 2429865
DOI: 10.1016/0014-5793(86)81489-2

Abstract

dNTP(3'-OCH3), a 3'-O-methyl derivative of dNTP, is a chain terminator substrate for DNA synthesis catalyzed by AMV reverse transcriptase. The enzyme seems to be the only DNA polymerase susceptible to the inhibitor while all the other DNA polymerases tested are fully resistant to the nucleotide analog. The resistant polymerases are: E. coli DNA polymerase I, Klenow's fragment of DNA polymerase I, phage T4 DNA polymerase, calf thymus DNA polymerase alpha, rat liver DNA polymerase beta and calf thymus terminal deoxyribonucleotidyl transferase.

MeSH terms

Animals
Cattle
DNA / biosynthesis
DNA Polymerase I / antagonists & inhibitors
DNA Polymerase II / antagonists & inhibitors
DNA-Directed DNA Polymerase / metabolism
Deoxyribonucleotides / pharmacology*
Escherichia coli / enzymology
Liver / enzymology
Nucleic Acid Synthesis Inhibitors
Oligonucleotides / metabolism
Rats
Reverse Transcriptase Inhibitors*
T-Phages / enzymology
Thymus Gland / enzymology

Substances

Deoxyribonucleotides
Nucleic Acid Synthesis Inhibitors
Oligonucleotides
Reverse Transcriptase Inhibitors
DNA
DNA Polymerase I
DNA Polymerase II
DNA-Directed DNA Polymerase