Abstract
Here, a 1.82 Å resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an Rfree of 0.196. Structural analysis shows that five related structures have been reported with a secondary-structure similarity of between 75 and 89%. The five similar structures were all either Clostridium thermocellum or Geotrichum sp. M128 GH74 xyloglucanases. Structural analysis indicates that the A. cellulolyticus GH74 enzyme is an endoxyloglucanase, as it lacks a characteristic loop that blocks one end of the active site in exoxyloglucanases. Superimposition with the C. thermocellum GH74 shows that Asp451 and Asp38 are the catalytic residues.
Keywords:
GH74; endoglucanases; glycoside hydrolases; xyloglucanases.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Actinomycetales / chemistry*
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Actinomycetales / enzymology
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Actinomycetales / genetics
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Amino Acid Sequence
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Clostridium thermocellum / chemistry
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Clostridium thermocellum / enzymology
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Clostridium thermocellum / genetics
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Crystallography, X-Ray
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Geotrichum / chemistry
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Geotrichum / enzymology
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Geotrichum / genetics
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Glycoside Hydrolases / chemistry*
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Glycoside Hydrolases / genetics
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Glycoside Hydrolases / metabolism
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Models, Molecular*
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Molecular Sequence Data
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Protein Interaction Domains and Motifs
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Protein Structure, Secondary
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Alignment
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Structural Homology, Protein
Substances
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Bacterial Proteins
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Recombinant Proteins
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Glycoside Hydrolases
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xyloglucan endo(1-4)-beta-D-glucanase