Abstract
Feline leukemia virus (FeLV) induces neoplastic and nonneoplastic diseases in cats. The transduction of cellular genes by FeLV is sometimes observed and associated with neoplastic diseases including lymphoma and sarcoma. Here, we report the first natural case of feline Notch2 transduction by FeLV in an infected cat with multicentric lymphoma and hypercalcemia. We cloned recombinant FeLVs harboring Notch2 in the env gene. Notch2 was able to activate expression of a reporter gene, similar to what was previously reported in cats with experimental FeLV-induced thymic lymphoma. Our findings suggest that the transduction of Notch2 strongly correlates with FeLV-induced lymphoma.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cat Diseases / genetics*
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Cat Diseases / virology*
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Cats
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Cloning, Molecular
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DNA Primers / genetics
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Female
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Gene Components
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Genes, env / genetics
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HEK293 Cells
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Humans
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Leukemia Virus, Feline / genetics
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Leukemia Virus, Feline / physiology*
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Leukemia, Feline / genetics*
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Leukemia, Feline / virology
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Luciferases
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Molecular Sequence Data
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Polymerase Chain Reaction / veterinary
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Receptor, Notch2 / genetics
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Receptor, Notch2 / metabolism*
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Sequence Alignment
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Sequence Analysis, DNA / veterinary
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Transduction, Genetic / veterinary*
Substances
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DNA Primers
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Receptor, Notch2
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Luciferases