[Comparison of ELISA diagnostic kits used in China for hepatitis C virus IgG antibody detection]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Jun;27(3):221-3.
[Article in Chinese]

Abstract

Objective: To compare the commercial diagnostic reagent available in China for hepatitis C virus ( HCV) IgG antibody detection in order to provide some useful information for HCV prevention.

Methods: The HCV-IgG detection reagents produced by six different Enterprises named A, B, C, D, E and F were chosen and applied to detect 160 HCV specious sera samples. HCV-IgG reagent from ABBOTT was adopted as gold-standard and the samples in gray zone were determined by RIBA method finally.

Results: 160 sera samples comprised 88 positive samples and 72 negative samples. The total conformity ranged from 88.13% to 98.13% and the Youden indexes ranged from 0.74 to 0.96 when the reagents from six different Enterprises were compared with gold-standard. The highest conformity was 98.13%, observed in D reagent with the highest Youden index of 0.96.

Conclusion: The total conformity rates were more than 88% when the HCV-IgG antibody detection reagents from six different Enterprises were compared with ABBOTT reagent. It was highly conformable. However, some reagent proved to be less conformable in negative samples detection.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Antibodies, Viral / blood*
  • China
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Hepacivirus / immunology*
  • Humans
  • Immunoglobulin G / blood*
  • Reagent Kits, Diagnostic*

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Reagent Kits, Diagnostic