Abstract
The 33 genes in the Saccharomyces cerevisiae mitotic CLB2 transcription cluster have been known to be downregulated by the DNA damage checkpoint for many years. Here, we show that this is mediated by the checkpoint kinase Rad53 and the dedicated transcriptional activator of the cluster, Ndd1. Ndd1 is phosphorylated in response to DNA damage, which blocks recruitment to promoters and leads to the transcriptional downregulation of the CLB2 cluster. Finally, we show that downregulation of Ndd1 is an essential function of Rad53, as a hypomorphic ndd1 allele rescues RAD53 deletion.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Checkpoint Kinase 2 / genetics
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Checkpoint Kinase 2 / metabolism*
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DNA Damage / genetics
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DNA Damage / physiology
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Down-Regulation / genetics
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Down-Regulation / physiology
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Gene Expression Regulation, Fungal / genetics*
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Gene Expression Regulation, Fungal / physiology
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Humans
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Mitosis*
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Promoter Regions, Genetic / genetics
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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Cell Cycle Proteins
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NDD1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Checkpoint Kinase 2
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RAD53 protein, S cerevisiae