Risk factors associated with preoperative fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in liver transplant recipients

Transpl Infect Dis. 2014 Feb;16(1):84-9. doi: 10.1111/tid.12169. Epub 2013 Dec 16.

Abstract

Objective: The aim of the study was to identify risk factors associated with pre-transplant fecal carriage of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in liver transplant recipients.

Patients and methods: Over a 3-year period (January 2009-December 2011), 317 patients who underwent liver transplantation were screened preoperatively for fecal carriage of ESBL-producing Enterobacteriaceae. Risk factors for fecal carriage were investigated by univariate analysis and stepwise logistic regression.

Results: Of the 317 patients screened, 50 (15.7%) harbored an ESBL-producing isolate. Previous infection with an ESBL-producing organism had developed during the last 6 months in 20% of fecal carriers versus in none of the non-carriers. Other variables associated with fecal carriage were a model for end-stage liver disease score ≥25, pre-transplant stay in the intensive care unit ≥48 h, hospital stay ≥10 days in the last 6 months, a history of spontaneous bacterial peritonitis (SBP), exposure to a β-lactam agent in the last month, and prophylaxis with norfloxacin. Independent predictors of fecal carriage in the multivariate logistic regression model were exposure to a β-lactam agent in the month preceding transplantation (odds ratio [OR] = 7.8, confidence interval [CI] = 4-15.5, P < 0.001), and a history of SBP (OR = 2.4, CI = 1.1-4.9, P = 0.02).

Conclusions: Previous infection with an ESBL-producing isolate, recent exposure to a β-lactam agent, and a history of SBP are risk factors for preoperative fecal carriage of ESBL-producing Enterobacteriaceae in liver transplant recipients. Patients at risk of fecal carriage should receive intraoperative prophylaxis and, when necessary, empiric postoperative antimicrobial treatment that includes coverage for these organisms.

Keywords: ESBL; Enterobacteriaceae; extended-spectrum β-lactamase; fecal carriage; liver transplant; spontaneous bacterial peritonitis.

MeSH terms

  • Adult
  • Amikacin
  • Cefoxitin
  • Ciprofloxacin
  • Drug Resistance, Bacterial
  • End Stage Liver Disease / complications
  • End Stage Liver Disease / surgery*
  • Enterobacter cloacae / isolation & purification
  • Enterobacter cloacae / physiology
  • Enterobacteriaceae / isolation & purification*
  • Enterobacteriaceae / physiology
  • Enterobacteriaceae Infections / complications
  • Enterobacteriaceae Infections / microbiology*
  • Escherichia coli / isolation & purification
  • Escherichia coli / physiology
  • Escherichia coli Infections / complications
  • Escherichia coli Infections / microbiology
  • Feces / microbiology*
  • Female
  • Humans
  • Imipenem
  • Klebsiella / isolation & purification
  • Klebsiella / physiology
  • Klebsiella Infections / complications
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / isolation & purification
  • Klebsiella pneumoniae / physiology
  • Liver Transplantation*
  • Logistic Models
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Multivariate Analysis
  • Penicillanic Acid / analogs & derivatives
  • Peritonitis
  • Piperacillin
  • Piperacillin, Tazobactam Drug Combination
  • Preoperative Period*
  • Risk Factors
  • Severity of Illness Index
  • beta-Lactamases / metabolism*
  • beta-Lactams*

Substances

  • beta-Lactams
  • Piperacillin, Tazobactam Drug Combination
  • Ciprofloxacin
  • Cefoxitin
  • Imipenem
  • Amikacin
  • Penicillanic Acid
  • beta-Lactamases
  • Piperacillin