A higher frequency of CD4⁺CXCR5⁺ T follicular helper cells in patients with newly diagnosed IgA nephropathy

Immunol Lett. 2014 Mar-Apr;158(1-2):101-8. doi: 10.1016/j.imlet.2013.12.004. Epub 2013 Dec 12.

Abstract

The frequency of different subsets of CD4(+)CXCR5(+) TFH cells and the levels of serum cytokines were examined in 24 patients with newly diagnosed IgAN before and 8-12 weeks after treatment and in 12 healthy controls (HC). In comparison with HC, significantly higher percentages of CD4(+)CXCR5(+), CD4(+)CXCR5(+)ICOS(+), CD4(+)CXCR5(+)PD-1(+) TFH cells and higher levels of serum IL-17A, IFN-γ, IL-2, IL-10, IL-4 and IL-21 were detected in IgAN patients. The percentages of CD4(+)CXCR5(+) TFH cells were negatively correlated with the values of eGFR and CD4(+)CXCR5(+)PD-1(+) TFH cells were correlated positively with the levels of serum IL-21 and Gd-IgA1 as well as 24h urinary proteins. Treatment with prednisone significantly reduced the frequency of CD4(+)CXCR5(+) and CD4(+)CXCR5(+)PD-1(+) TFH cells and the levels of serum IL-21, but increased IL-4 and IL-10 in those patients. A higher frequency of CD4(+)CXCR5(+) TFH cells existed in patients with IgAN and may be associated with the development of IgAN.

Keywords: IgA nephropathy; Prednisone; TFH cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • CD4 Antigens / metabolism
  • Coordination Complexes
  • Cytokines / blood
  • Female
  • Glomerulonephritis, IGA / diagnosis
  • Glomerulonephritis, IGA / drug therapy*
  • Glomerulonephritis, IGA / immunology*
  • Humans
  • Immunoglobulin A
  • Immunophenotyping
  • Lymphocyte Activation / drug effects
  • Male
  • Middle Aged
  • Prednisone / therapeutic use*
  • Programmed Cell Death 1 Receptor / metabolism
  • Receptors, CXCR5 / metabolism
  • T-Lymphocyte Subsets / drug effects
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocytes, Helper-Inducer / drug effects
  • T-Lymphocytes, Helper-Inducer / immunology*
  • Th1 Cells / immunology*
  • Th17 Cells / immunology*
  • Young Adult

Substances

  • CD4 Antigens
  • CXCR5 protein, human
  • Coordination Complexes
  • Cytokines
  • Immunoglobulin A
  • PDCD1 protein, human
  • Programmed Cell Death 1 Receptor
  • Receptors, CXCR5
  • Prednisone