Protective effect of aquacultured flounder fish-derived peptide against oxidative stress in zebrafish

Fish Shellfish Immunol. 2014 Jan;36(1):320-3. doi: 10.1016/j.fsi.2013.11.018. Epub 2013 Dec 11.

Abstract

This study investigates the protective effect of aquacultured flounder fish-derived peptide (AFFP) against 2,2-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced oxidative damage in a zebrafish model. Zebrafish embryos were evaluated for the protective effect by heartbeat rate, survival rate, ROS generation, lipid peroxidation, and cell death. In the results, the AAPH group showed a low survival rate, whereas the AFFP and AAPH co-treated group increased a survival rate. Also, AFFP dose-dependently reduced AAPH-induced intracellular ROS and lipid peroxidation, and decreased cell death in AAPH-induced zebrafish. These results revealed that AFFP could be used as a natural antioxidant, and that the zebrafish provides an alternative in vivo model to efficiently evaluate the antioxidative effects of peptides on fishes.

Keywords: AAPH; Antioxidative peptide; In vivo model; Oxidative stress; Zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidines / administration & dosage
  • Animals
  • Cell Death / immunology
  • Heart Rate / immunology
  • Lipid Peroxidation / immunology
  • Oxidative Stress / immunology*
  • Peptides / pharmacology*
  • Random Allocation
  • Reactive Oxygen Species / immunology*
  • Spectrometry, Fluorescence
  • Zebrafish / immunology*

Substances

  • Amidines
  • Peptides
  • Reactive Oxygen Species
  • 2,2'-azobis(2-amidinopropane)