Whole blood cells loaded with messenger RNA as an anti-tumor vaccine

Adv Healthc Mater. 2014 Jun;3(6):837-42. doi: 10.1002/adhm.201300512. Epub 2013 Dec 16.

Abstract

The use of a cell-based vaccine composed of autologous whole blood cells loaded with mRNA is described. Mice immunized with whole blood cells loaded with mRNA encoding antigen develop anti-tumor immunity comparable to DC-RNA immunization. This approach offers a simple and affordable alternative to RNA-based cellular therapy by circumventing complex, laborious and expensive ex vivo manipulations required for DC-based immunizations.

Keywords: blood; cancer; immunotherapy; messenger RNA; vaccine.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / immunology
  • Antigens, Neoplasm / metabolism
  • Blood Cells / cytology
  • Blood Cells / metabolism*
  • Blood Cells / transplantation
  • Cancer Vaccines / genetics
  • Cancer Vaccines / immunology*
  • Cancer Vaccines / metabolism
  • Dendritic Cells / cytology
  • Dendritic Cells / metabolism
  • Dendritic Cells / transplantation
  • Disease Models, Animal
  • Electroporation
  • Female
  • Immunotherapy
  • Intramolecular Oxidoreductases / genetics
  • Intramolecular Oxidoreductases / metabolism
  • Melanoma, Experimental / mortality
  • Melanoma, Experimental / pathology
  • Melanoma, Experimental / therapy
  • Mice
  • Mice, Inbred C57BL
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism*
  • Survival Rate

Substances

  • Antigens, Neoplasm
  • Cancer Vaccines
  • RNA, Messenger
  • Intramolecular Oxidoreductases
  • dopachrome isomerase