Abstract
Exchange of the extremities of the NS segment of type A and C influenza viruses in reverse genetics systems was used to assess their putative role in type specificity. Restoration of each specific proximal panhandle was mandatory to allow the rescue of viruses with heterotypic extremities. Moreover, the transcription level of the modified segment seemed to be directly affected by the distal panhandle strength.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Gammainfluenzavirus / genetics
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Gammainfluenzavirus / metabolism*
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Humans
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Influenza A virus / genetics
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Influenza A virus / metabolism*
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Viral Nonstructural Proteins / chemistry*
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Viral Nonstructural Proteins / genetics
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Viral Nonstructural Proteins / metabolism*
Substances
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Viral Nonstructural Proteins
Grants and funding
This work was supported by institutional funds from the Institut Pasteur, the Centre National de la Recherche Scientifique and the University Paris Diderot, Sorbonne Paris Cité, Paris, France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.