Histological subtypes and characteristic structures of HPV-associated oropharyngeal carcinoma: study with Japanese cases

Diagn Pathol. 2013 Dec 19:8:211. doi: 10.1186/1746-1596-8-211.

Abstract

Background: Human papillomavirus-associated oropharyngeal carcinoma (HPV-OPC) is clinicopathologically distinct entity from the HPV-unassociated one (nHPV-OPC). This study aimed to determine the relationship between histological subtypes of OPC and HPV status for Japanese cases and to identify histological structures of HPV-OPC.

Methods: 66 OPC cases were categorized into conventional squamous cell carcinoma (SCC) and the variants. Conventional SCC was subcategorized into keratinizing (KSCC), non-keratinizing (NKSCC), and hybrid SCC (HSCC). HPV status of all cases was determined using p16-immunohistochemistry and HPV-DNA ISH.

Results: Two histological subtypes, NKSCC and HSCC, tended to be HPV-OPC and KSCC tended to be nHPV-OPC with statistical significance. Two histological structures, abrupt keratinization, defined in the text, and comedo-necrosis among non-maturing tumor island, were observed for 58.1% and 38.7% of HPV-OPC, and tended to exist for HPV-OPC with statistical significance.

Conclusions: This study showed the association of NKSCC/HSCC with HPV-OPC in Japanese cases, and two histological structures, abrupt keratinization and comedo-necrosis among non-maturing island, were considered characteristic histological features of HPV-OPC.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1816432541113073.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Asian People*
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Squamous Cell / classification*
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / virology*
  • Cyclin-Dependent Kinase Inhibitor p16 / metabolism
  • DNA, Viral / genetics
  • Female
  • Humans
  • In Situ Hybridization
  • Japan
  • Male
  • Middle Aged
  • Oropharyngeal Neoplasms / classification*
  • Oropharyngeal Neoplasms / pathology
  • Oropharyngeal Neoplasms / virology*
  • Oropharynx / metabolism
  • Oropharynx / pathology
  • Papillomaviridae / genetics
  • Papillomavirus Infections / complications*
  • Retrospective Studies

Substances

  • Biomarkers, Tumor
  • Cyclin-Dependent Kinase Inhibitor p16
  • DNA, Viral