An oligofluorene truxene based distributed feedback laser for biosensing applications

Anne-Marie Haughey; Benoit Guilhabert; Alexander L Kanibolotsky; Peter J Skabara; Martin D Dawson; Glenn A Burley; Nicolas Laurand

doi:10.1016/j.bios.2013.11.054

An oligofluorene truxene based distributed feedback laser for biosensing applications

Biosens Bioelectron. 2014 Apr 15:54:679-86. doi: 10.1016/j.bios.2013.11.054. Epub 2013 Nov 25.

Authors

Anne-Marie Haughey¹, Benoit Guilhabert², Alexander L Kanibolotsky³, Peter J Skabara⁴, Martin D Dawson⁵, Glenn A Burley⁶, Nicolas Laurand⁷

Affiliations

¹ Institute of Photonics, SUPA, University of Strathclyde, Glasgow G4 0NW, UK. Electronic address: [email protected].
² Institute of Photonics, SUPA, University of Strathclyde, Glasgow G4 0NW, UK. Electronic address: [email protected].
³ WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow G1 1XL, UK. Electronic address: [email protected].
⁴ WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow G1 1XL, UK. Electronic address: [email protected].
⁵ Institute of Photonics, SUPA, University of Strathclyde, Glasgow G4 0NW, UK. Electronic address: [email protected].
⁶ WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow G1 1XL, UK. Electronic address: [email protected].
⁷ Institute of Photonics, SUPA, University of Strathclyde, Glasgow G4 0NW, UK. Electronic address: [email protected].

PMID: 24355421
DOI: 10.1016/j.bios.2013.11.054

Abstract

The first example of an all-organic oligofluorene truxene based distributed feedback laser for the detection of a specific protein-small molecule interaction is reported. The protein avidin was detected down to 1 μg mL(-1) using our biotin-labelled biosensor platform. This interaction was both selective and reversible when biotin was replaced with desthiobiotin. Avidin detection was not perturbed by Bovine Serum Albumin up to 50,000 μg mL(-1). Our biosensor offers a new detection platform that is both highly sensitive, modular and potentially re-usable.

Keywords: Biosensor; Laser; Oligofluorene truxene; Organic.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Avidin / analysis*
Avidin / metabolism*
Biosensing Techniques / instrumentation*
Biotin / analogs & derivatives
Biotin / metabolism*
Biotinylation
Cattle
Equipment Design
Fluorenes / chemistry*
Lasers*
Semiconductors
Sensitivity and Specificity
Serum Albumin, Bovine / metabolism

Substances

Fluorenes
Avidin
Serum Albumin, Bovine
Biotin
desthiobiotin