Fish cholinesterases as biomarkers of sublethal effects of organophosphorus and carbamates in tissues of Labeo rohita

J Biochem Mol Toxicol. 2014 Mar;28(3):137-42. doi: 10.1002/jbt.21545. Epub 2013 Dec 19.

Abstract

Organophosphates and carbamates are major agrochemicals that strongly affect different neuroenzymes and the growth of various fish species. Here, we study the effect of sublethal concentrations of profenofos and carbofuran on the activity of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) and the associated health risk in fish. Labeo rohita fingerlings were exposed to three sublethal concentrations of profenofos and carbofuran. The minimum cholinesterase activities in the brain, gills, muscle, kidney, liver, and blood were after exposure to profenofos (0.06 mg/L). The minimum AChE and BuChE activities in the brain, gills, muscle, kidney, liver, and blood were after exposure to carbofuran (0.28 and 0.198 mg/L). Exposure to both types of pesticides affected the functions of these organs, including metabolism and neurotransmission, to various extents at different exposure concentrations. These findings suggest that they are required to be properly monitored in the environment, to reduce their toxic effects on nontarget organisms.

Keywords: Acetylcholine-sterase; Butyrylcholinesterase; Carbofuran; Inhibition; Profenofos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / metabolism*
  • Animals
  • Biomarkers / analysis*
  • Biomarkers / metabolism
  • Brain / drug effects
  • Brain / enzymology
  • Butyrylcholinesterase / metabolism*
  • Carbamates / toxicity
  • Carbofuran / toxicity*
  • Cyprinidae / metabolism*
  • Dose-Response Relationship, Drug
  • Organothiophosphates / toxicity*
  • Pesticides / toxicity
  • Toxicity Tests
  • Water Pollutants, Chemical / toxicity

Substances

  • Biomarkers
  • Carbamates
  • Organothiophosphates
  • Pesticides
  • Water Pollutants, Chemical
  • profenofos
  • Acetylcholinesterase
  • Butyrylcholinesterase
  • Carbofuran