Identification of the novel lincosamide resistance gene lnu(E) truncated by ISEnfa5-cfr-ISEnfa5 insertion in Streptococcus suis: de novo synthesis and confirmation of functional activity in Staphylococcus aureus

Antimicrob Agents Chemother. 2014;58(3):1785-8. doi: 10.1128/AAC.02007-13. Epub 2013 Dec 23.

Abstract

The novel lincosamide resistance gene lnu(E), truncated by insertion of an ISEnfa5-cfr-ISEnfa5 segment, was identified in Streptococcus suis. The gene lnu(E) encodes a 173-amino-acid protein with ≤69.4% identity to other lincosamide nucleotidyltransferases. The lnu(E) gene and its promoter region were de novo synthesized, and Staphylococcus aureus RN4220 carrying a shuttle vector with the cloned lnu(E) gene showed a 16-fold increase in the lincomycin MIC. Mass spectrometry experiments demonstrated that Lnu(E) catalyzed the nucleotidylation of lincomycin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • Cloning, Molecular
  • Drug Resistance, Bacterial / genetics
  • Genes, Bacterial / genetics
  • INDEL Mutation / genetics
  • Lincosamides / pharmacology*
  • Molecular Sequence Data
  • R Factors / chemical synthesis
  • R Factors / genetics*
  • Sequence Alignment
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics*
  • Streptococcus suis / drug effects
  • Streptococcus suis / genetics*

Substances

  • Anti-Bacterial Agents
  • Lincosamides

Associated data

  • GENBANK/KF287643