First report on the occurrence of Theileria sp. OT3 in China

Zhancheng Tian; Guangyuan Liu; Hong Yin; Junren Xie; Suyan Wang; Xiaosong Yuan; Fangfang Wang; Jin Luo

doi:10.1016/j.parint.2013.12.014

First report on the occurrence of Theileria sp. OT3 in China

Parasitol Int. 2014 Apr;63(2):403-7. doi: 10.1016/j.parint.2013.12.014. Epub 2014 Jan 3.

Authors

Zhancheng Tian¹, Guangyuan Liu², Hong Yin³, Junren Xie³, Suyan Wang³, Xiaosong Yuan³, Fangfang Wang³, Jin Luo³

Affiliations

¹ Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China. Electronic address: [email protected].
² Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China. Electronic address: [email protected].
³ Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.

PMID: 24388914
DOI: 10.1016/j.parint.2013.12.014

Abstract

Theileria sp. OT3 was firstly detected and identified from clinically healthy sheep in Xinjiang Uygur Autonomous Region of China (XUAR) through comparing the complete 18S rDNA gene sequences available in GenBank database and the phylogenetic status based on the internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene by the methods of a partitioned multi-locus analysis in BEAST and Maximum likelihood analysis in PhyML. Moreover, the findings were confirmed by the species-specific PCR for Theileria sp. OT3 and the prevalence of Theileria sp. OT3 was 14.9% in the north of XUAR. This study is the first report on the occurrence of Theileria sp. OT3 in China.

Keywords: 18S rDNA; ITS1–5.8S gene–ITS2 region; Species-specific PCR; Theileria sp. OT3; Tick.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
China / epidemiology
Molecular Sequence Data
Phylogeny
Sheep
Sheep Diseases / epidemiology
Sheep Diseases / parasitology*
Theileria / classification*
Theileria / isolation & purification*
Theileriasis / epidemiology
Theileriasis / parasitology*