Secretome analysis of human oligodendrocytes derived from neural stem cells

Woo Kyung Kim; Deokhoon Kim; Jun Cui; Ho Hee Jang; Kwang Sei Kim; Hong Jun Lee; Seung U Kim; Sung-Min Ahn

doi:10.1371/journal.pone.0084292

Secretome analysis of human oligodendrocytes derived from neural stem cells

PLoS One. 2014 Jan 2;9(1):e84292. doi: 10.1371/journal.pone.0084292. eCollection 2014.

Authors

Woo Kyung Kim¹, Deokhoon Kim², Jun Cui³, Ho Hee Jang³, Kwang Sei Kim⁴, Hong Jun Lee⁴, Seung U Kim⁵, Sung-Min Ahn⁶

Affiliations

¹ Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Korea ; Asan Center for Cancer Genome Discovery, Asan Institute for Life Science, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea ; BRC Genome Research Center, Bio Research Complex, Incheon, Korea.
² Asan Center for Cancer Genome Discovery, Asan Institute for Life Science, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea.
³ Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Korea.
⁴ Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea.
⁵ Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea ; Division of Neurology, Department of Medicine, UBC Hospital, University of British Columbia, Vancouver, British Columbia, Canada.
⁶ Asan Center for Cancer Genome Discovery, Asan Institute for Life Science, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea ; Department of Oncology, Ulsan University College of Medicine, Seoul, Korea.

Abstract

In this study, we investigated the secretome of human oligodendrocytes (F3.Olig2 cells) generated from human neural stem cells by transduction with the gene encoding the Olig2 transcription factor. Using mRNA sequencing and protein cytokine arrays, we identified a number of biologically important secretory proteins whose expression has not been previously reported in oligodendrocytes. We found that F3.Olig2 cells secrete IL-6, PDGF-AA, GRO, GM-CSF, and M-CSF, and showed prominent expression of their corresponding receptors. Co-expression of ligands and receptors suggests that autocrine signaling loops may play important roles in both differentiation and maintenance of oligodendrocytes. We also found that F3.Olig2 cells secrete matrix metalloproteinases and matrix metalloproteinase-associated proteins associated with functional competence of oligodendrocytes. The results of our secretome analysis provide insights into the functional and molecular details of human oligodendrocytes. To the best of our knowledge, this is the first systematic analysis of the secretome of oligodendrocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Basic Helix-Loop-Helix Transcription Factors / genetics
Basic Helix-Loop-Helix Transcription Factors / metabolism
Biomarkers
Cell Differentiation* / genetics
Cytokines / genetics
Cytokines / metabolism
Gene Expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Humans
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Neural Stem Cells / cytology*
Neural Stem Cells / metabolism
Oligodendrocyte Transcription Factor 2
Oligodendroglia / cytology*
Oligodendroglia / metabolism*
Organ Specificity / genetics
Proteome*
Proteomics* / methods
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
Signal Transduction
Transduction, Genetic

Substances

Basic Helix-Loop-Helix Transcription Factors
Biomarkers
Cytokines
Nerve Tissue Proteins
OLIG2 protein, human
Oligodendrocyte Transcription Factor 2
Proteome
Receptors, Cell Surface

Grants and funding

This research was supported by basic science research program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2011-0014717). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.