Poly(3-hydroxybutyrate-co-R-3-hydroxyhexanoate) nanoparticles with polyethylenimine coat as simple, safe, and versatile vehicles for cell targeting: population characteristics, cell uptake, and intracellular trafficking

Adv Healthc Mater. 2014 Jun;3(6):817-24. doi: 10.1002/adhm.201300533. Epub 2014 Jan 10.

Abstract

A simple and highly safe poly(3-hydroxybutyrate-co-R-3-hydroxyhexanoate) nanoparticulate delivery system that targets different cell types is developed. A sub-cytotoxic level of polyethylenimine coat mediates universal cell targeting. Internalized nanoparticles traffic along endolysosomal compartments, endoplasmic reticulum and the Golgi complex. Nanoparticles have no detrimental effects on cell morphology and respiration.

Keywords: cell targeting; intracellular trafficking; polyethylenimine; polyhydroxyalkanoate; surface coating.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Hydroxybutyric Acid / chemistry*
  • Biological Transport
  • Caproates / chemistry*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Humans
  • Microscopy, Confocal
  • Nanoparticles / chemistry*
  • Nanoparticles / metabolism
  • Nanoparticles / toxicity
  • Oxidative Phosphorylation / drug effects
  • Particle Size
  • Polyethyleneimine / chemistry*
  • Rhodamines / chemistry
  • Rhodamines / metabolism

Substances

  • Caproates
  • Rhodamines
  • poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
  • Polyethyleneimine
  • rhodamine B
  • 3-Hydroxybutyric Acid