The typical characteristics of mesenchymal stem cells (MSCs) can be affected by inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from HTLV-1-infected symptomatic individuals compared with those cultured with cells from asymptomatic infected individuals or healthy subjects. HTLV-1 does not impair the MSC differentiation process into osteocytes and adipocytes. In addition, MSCs were efficiently infected with HTLV-1 in vitro through direct contact with HTLV-1-infected cells; however, cell-free virus particles were not capable of causing infection. In summary, HTLV-1 can alter MSC function, and this mechanism may contribute to the pathogenesis of this viral infection.
Keywords: ACTB; ATLL; BM; CD105; CD106; CD13; CD271; CD45; CD73; CD90; CMV; ENG, endoglin; GAPDH; GLUT1; HAC; HAM/TSP; HHV; HLA-ABC; HLA-class I; HSPG2; HTLV asymptomatic carriers; HTLV-1; HTLV-associated myelopathy/tropical spastic paraparesis; Human mesenchymal stromal cells; MSC; MSC differentiation; MSC virus infection; NGFR, nerve growth factor receptor; NRP1; NT5E, 5-nucleotidase, ecto; PBMC; PPARγ; RUNX-2; THY1, Thy-1 cell surface antigen; URE; VCAM1, vascular cell adhesion molecule 1; adult T-cell leukemia/lymphoma; aminopeptidase-N; beta-actin; bone marrow; cytomegalovirus; expression relative units; glyceraldehyde-3-phosphate dehydrogenase; heparan sulfate proteoglycan 2; human herpesvirus, HHV8; human major histocompatibility complex (MHC) class I, HLA-A, B, C; human major histocompatibility complex, class I; leukocyte common; mesenchymal stromal cells; neuropilins 1; peripheral blood mononuclear cells; peroxisome proliferator-activated receptors gamma; runt-related transcription factor-2; solute carrier family 2 (facilitated glucose transporter), member 1.
© 2013 Published by Elsevier Inc.