Absence of cytokeratin in human hepatoma cell lines

Cell Biol Int Rep. 1987 Jun;11(6):477-86. doi: 10.1016/0309-1651(87)90081-6.

Abstract

The immunofluorescence study revealed that both our established human hepatoma cell lines, HA22T/VGH and HA47T/VGH, were absent of cytokeratin. This observation was further confirmed by a western blot study. However, they as well as the other human hepatoma cells, Hep G2, Hep 3B, and SK-Hep-1 expressed vimentin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Hepatocellular / analysis*
  • Cell Line
  • Cytoskeleton / analysis*
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Humans
  • Immunoassay
  • Intermediate Filaments / analysis*
  • Keratins / analysis*
  • Liver Neoplasms / analysis*
  • Vimentin / analysis

Substances

  • Vimentin
  • Keratins