Mucosal and systemic immunity in mice after intranasal immunization with recombinant Lactococcus lactis expressing ORF6 of PRRSV

Zhen-hua Wang; Xiao-han Cao; Xiao-gang Du; Hai-bo Feng; Di-Wang; He-Song; Xian-yin Zeng

doi:10.1016/j.cellimm.2013.12.004

Mucosal and systemic immunity in mice after intranasal immunization with recombinant Lactococcus lactis expressing ORF6 of PRRSV

Cell Immunol. 2014 Feb;287(2):69-73. doi: 10.1016/j.cellimm.2013.12.004. Epub 2013 Dec 17.

Authors

Zhen-hua Wang¹, Xiao-han Cao², Xiao-gang Du², Hai-bo Feng³, Di-Wang¹, He-Song¹, Xian-yin Zeng⁴

Affiliations

¹ Department of Animal and Veterinary Science, Chengdu Vocational College of Agricultural Science and Technology, WenJiang, Sichuan 611130, PR China.
² Isotope Research Laboratory, College of Life and Basic Sciences, Sichuan Agricultural University, Xin Kang Road 46, Ya'an, Sichuan 625014, PR China.
³ Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
⁴ Isotope Research Laboratory, College of Life and Basic Sciences, Sichuan Agricultural University, Xin Kang Road 46, Ya'an, Sichuan 625014, PR China. Electronic address: [email protected].

PMID: 24423464
DOI: 10.1016/j.cellimm.2013.12.004

Abstract

The purpose of the study was to construct mucosal vaccine of a recombinant Lactococcus lactis expressing PRRSV ORF6 gene and evaluate mucosal and systemic immune response against PRRSV in mice after intranasal immunization. The result show that the vaccine can stimulate mice to produce specific IgG in serum and remarkable special s-IgA in lung lavage fluid, at the same time, the contents of cytokines IL-2 and IFN-γ of the experimental group were significant higher than those of the control group (P < 0.01), however, the contents of cytokines IL-4 was not different to the all groups. In summary, the constructed mucosal vaccine can significantly induce mucosal immune, humoral immunity and cellular immunity involved Th1 type cytokines, which will lay a theoretical foundation on immune mechanism and new efficient vaccines for PRRSV.

Keywords: Intranasal immunization; Lactococcus lactis; Mucosal immunity; PRRSV-ORF6.

MeSH terms

Administration, Intranasal
Animals
Bronchoalveolar Lavage Fluid / immunology
Cells, Cultured
Cytokines / immunology
Female
Genetic Engineering
Genetic Vectors
Immunity, Mucosal
Immunoglobulin A / blood
Immunoglobulin G / blood
Lactococcus lactis
Mice
Mice, Inbred BALB C
Nasal Mucosa / immunology*
Nisin / metabolism
Open Reading Frames / genetics
Open Reading Frames / immunology
Porcine Reproductive and Respiratory Syndrome / prevention & control*
Porcine respiratory and reproductive syndrome virus / immunology*
Recombinant Proteins / biosynthesis
Recombinant Proteins / genetics
Recombinant Proteins / immunology*
Swine
Th1 Cells / immunology
Th1-Th2 Balance
Vaccination / methods
Viral Proteins / biosynthesis
Viral Proteins / genetics
Viral Proteins / immunology*
Viral Vaccines / administration & dosage*
Viral Vaccines / genetics
Viral Vaccines / immunology*

Substances

Cytokines
Immunoglobulin A
Immunoglobulin G
Recombinant Proteins
Viral Proteins
Viral Vaccines
Nisin