Taxonomic and phenotypic characterization of yeasts isolated from worldwide cold rock-associated habitats

Fungal Biol. 2014 Jan;118(1):61-71. doi: 10.1016/j.funbio.2013.11.002. Epub 2013 Nov 26.

Abstract

Yeast strains isolated from rock samples collected from worldwide cold regions were identified by sequence analysis of the D1/D2 domains of the 26S rDNA gene and the ITS region followed by molecular phylogeny. Over 77% of yeasts isolates were Basidiomycota. Cryptococcus (orders Filobasidiales and Tremellales) and Rhodotorula (order Cystobasidiales) were the most frequent genera. About 40% of yeast isolates belonged to undescribed species. Almost all isolates were psychrotolerant. Urease and esterase were the most widely extracellular enzymatic activity at 4 °C and 20 °C. None of the strains exhibited extracellular protease, DNAse, cellulase, chitinase, and laccase activity. The taxonomic and ecological significance of yeasts associated to worldwide cold rocky habitats is discussed.

Keywords: D1/D2; Extreme environments; ITS; Lithic habitats; Phylogeny.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodiversity*
  • Cluster Analysis
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Environmental Microbiology*
  • Genes, rRNA
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Fungal / genetics
  • RNA, Ribosomal / genetics
  • Sequence Analysis, DNA
  • Yeasts / classification*
  • Yeasts / genetics
  • Yeasts / isolation & purification*

Substances

  • DNA, Fungal
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer
  • RNA, Fungal
  • RNA, Ribosomal
  • RNA, ribosomal, 26S

Associated data

  • GENBANK/JX092244
  • GENBANK/JX092245
  • GENBANK/JX092246
  • GENBANK/JX092247
  • GENBANK/JX092248
  • GENBANK/JX092249
  • GENBANK/JX092250
  • GENBANK/JX092251
  • GENBANK/JX092252
  • GENBANK/JX092253
  • GENBANK/JX092254
  • GENBANK/JX092255
  • GENBANK/JX092256
  • GENBANK/JX092257
  • GENBANK/JX092258
  • GENBANK/JX092259
  • GENBANK/JX092260
  • GENBANK/JX092261
  • GENBANK/JX092262
  • GENBANK/JX124713
  • GENBANK/JX124714
  • GENBANK/JX124715
  • GENBANK/JX124716
  • GENBANK/JX124717
  • GENBANK/JX124718
  • GENBANK/JX124719
  • GENBANK/JX124720
  • GENBANK/JX124721
  • GENBANK/JX124722
  • GENBANK/JX681815