Enterolactone concentrations and prognosis after postmenopausal breast cancer: assessment of effect modification and meta-analysis

Int J Cancer. 2014 Aug 15;135(4):923-33. doi: 10.1002/ijc.28729. Epub 2014 Feb 5.

Abstract

We previously reported that high concentrations of enterolactone, a lignan metabolite, are associated with lower mortality in 1,140 breast cancer patients from Germany. Using an extended set of 2,182 patients aged 50-74 years at diagnosis (2001-2005) and prospectively followed up until 2009, we investigated whether the association with mortality differs by lifestyle factors and tumor characteristics. Hazard ratios (HR) and 95% confidence intervals (CI) were estimated using multivariable Cox regression. Potential differential effects by tumor characteristics and lifestyle factors were assessed and a meta-analysis of five studies addressing lignan exposure and breast cancer prognosis was performed to summarize evidence. Median enterolactone concentrations were 17.4 (± 30.5 standard deviation) and 22.9 nmol L(-1) (± 44.8), respectively, for 269 deceased and 1,913 patients still alive. High enterolactone concentrations were significantly associated with lower all-cause mortality (per 10 nmol L(-1) : HR 0.94, 95% CI 0.90-0.98), breast cancer-specific mortality (HR 0.94, 0.89-0.99), and distant disease-free survival (HR 0.94, 0.90-0.98). Associations were found for stage 0-IIIA but not for stage IIIB-IV disease (p(het) = 0.01) and were stronger in patients with BMI <25 kg m(-2) than those with BMI ≥ 25 (p(het) = 0.04). In patients with healthy lifestyle (BMI <25, nonsmoker, physically active), the inverse association with all-cause mortality was still apparent (HR 0.92, 0.85-0.99). The meta-analysis yielded significant associations both for all-cause (HR 0.57, 0.42-0.78) and breast cancer-specific mortality (HR 0.54, 0.39-0.75). Our findings show that high lignan exposure is associated with reduced mortality in breast cancer patients. The inverse association observed in this study cannot be entirely explained by a healthy lifestyle.

Keywords: breast cancer mortality; enterolactone; lignans; phytoestrogens.

Publication types

  • Meta-Analysis
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Butyrolactone / analogs & derivatives*
  • 4-Butyrolactone / blood
  • Aged
  • Biomarkers, Tumor / blood
  • Body Mass Index
  • Breast Neoplasms / blood*
  • Breast Neoplasms / diagnosis
  • Breast Neoplasms / mortality
  • Diet*
  • Disease Progression
  • Disease-Free Survival
  • Female
  • Germany
  • Humans
  • Life Style
  • Lignans / blood*
  • Middle Aged
  • Postmenopause
  • Prognosis
  • Proportional Hazards Models
  • Prospective Studies
  • Risk Factors

Substances

  • Biomarkers, Tumor
  • Lignans
  • 4-Butyrolactone
  • 2,3-bis(3'-hydroxybenzyl)butyrolactone