Sema4A inhibits the therapeutic effect of IFN-β in EAE

J Neuroimmunol. 2014 Mar 15;268(1-2):43-9. doi: 10.1016/j.jneuroim.2013.12.014. Epub 2014 Jan 7.

Abstract

Approximately one-third of patients with multiple sclerosis (MS) respond poorly to interferon-beta (IFN-β) therapy. Serum Sema4A is increased in MS patients, and those who have high Sema4A do not respond to IFN-β therapy. In this study, we investigated whether recombinant Sema4A abrogates the efficacy of IFN-β in mice with experimental autoimmune encephalomyelitis (EAE), an animal model of MS. Administration of Sema4A concurrently with IFN-β diminished the efficacy of IFN-β in EAE. These effects of Sema4A were attributed to promote Th1 and Th17 differentiation and to increase adhesive activation of T cells to endothelial cells, even in the presence of IFN-β.

Keywords: Experimental autoimmune encephalomyelitis (EAE); Interferon-beta (IFN-β); Multiple sclerosis (MS); Sema4A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Differentiation / immunology
  • Drug Resistance
  • Encephalomyelitis, Autoimmune, Experimental / drug therapy
  • Encephalomyelitis, Autoimmune, Experimental / immunology
  • Encephalomyelitis, Autoimmune, Experimental / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Immunohistochemistry
  • Immunologic Factors / pharmacology*
  • Interferon-beta / pharmacology*
  • Lymphocyte Activation / drug effects*
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Inbred C57BL
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Semaphorins / metabolism*
  • Semaphorins / pharmacology

Substances

  • Immunologic Factors
  • Sema4A protein, mouse
  • Semaphorins
  • Interferon-beta