Evaluation of gelatin hydrogel as a potential carrier for cell transportation

Yoshimi Ohyabu; Hirosuke Hatayama; Shunji Yunoki

doi:10.1016/j.jbiosc.2013.12.005

Evaluation of gelatin hydrogel as a potential carrier for cell transportation

J Biosci Bioeng. 2014 Jul;118(1):112-5. doi: 10.1016/j.jbiosc.2013.12.005. Epub 2014 Jan 21.

Authors

Yoshimi Ohyabu¹, Hirosuke Hatayama², Shunji Yunoki²

Affiliations

¹ Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan. Electronic address: [email protected].
² Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

PMID: 24457148
DOI: 10.1016/j.jbiosc.2013.12.005

Abstract

We prepared uncleaved gelatin composed mainly of collagen α-, β-, and γ-chains. Gelation and melting of uncleaved gelatin occurred rapidly with moderate decrease and increase in temperature (23°C-37°C). The viability of cells encapsulated in the gelatin gel was greater than 96% after 7 d at 23°C.

Keywords: Cell carrier; Collagen; Gel; Gelatin; Renaturation; Transportation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Survival
Gelatin / chemistry*
Hydrogels / chemistry*
Mice
NIH 3T3 Cells
Preservation, Biological*
Temperature
Transportation

Substances

Hydrogels
Gelatin