Polymorphism of the CD14 and TLR4 genes and post-treatment apical periodontitis

J Endod. 2014 Feb;40(2):168-72. doi: 10.1016/j.joen.2013.10.006. Epub 2013 Nov 13.

Abstract

Introduction: This study investigated the association of CD14 -260C>T and TLR4 +896A>G gene polymorphisms with post-treatment apical periodontitis in Brazilian individuals.

Methods: The study population consisted of 41 patients with post-treatment apical periodontitis and 42 individuals with root canal-treated teeth exhibiting healed/healing periradicular tissues (controls). All teeth had apical periodontitis lesions at the time of treatment, which was completed at least 1 year previously. Saliva was collected from the participants; DNA was extracted and used for CD14 and TLR4 genotyping using the polymerase chain reaction-restriction fragment length polymorphism approach and a real-time polymerase chain reaction TaqMan assay (Applied Biosystems, Foster City, CA), respectively.

Results: No specific genotype or allele of the CD14 and TLR4 genes or any combination thereof was positively associated with post-treatment apical periodontitis (P > .05).

Conclusions: Data from the present study suggest that polymorphisms in the CD14 and TLR4 genes do not influence the response to endodontic treatment of teeth with apical periodontitis.

Keywords: CD14; TLR4; genetic polymorphism; post-treatment apical periodontitis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine
  • Adult
  • Cytosine
  • Female
  • Gene Frequency / genetics
  • Genotype
  • Guanine
  • Homozygote
  • Humans
  • Lipopolysaccharide Receptors / genetics*
  • Male
  • Periapical Periodontitis / genetics
  • Periapical Periodontitis / therapy*
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic / genetics*
  • Polymorphism, Restriction Fragment Length / genetics
  • Polymorphism, Single Nucleotide / genetics
  • Radiography, Bitewing / methods
  • Real-Time Polymerase Chain Reaction / methods
  • Root Canal Therapy / methods
  • Saliva / chemistry
  • Smoking
  • Thymine
  • Toll-Like Receptor 4 / genetics*
  • Wound Healing / genetics

Substances

  • Lipopolysaccharide Receptors
  • TLR4 protein, human
  • Toll-Like Receptor 4
  • Guanine
  • Cytosine
  • Adenine
  • Thymine