Abstract
Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N = 34) and APC in a series of unselected PCD (N = 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N = 14), SMM (N = 35) and MM (N = 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p = 0.0002).
Keywords:
CD81; Monoclonal gammopathy of uncertain significance; Multiple myeloma; Normal plasma cell immunophenotype; Smoldering multiple myeloma.
Published by Elsevier Ltd.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Bone Marrow / immunology
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Bone Marrow / pathology*
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CD56 Antigen / genetics
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CD56 Antigen / immunology
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Cell Differentiation
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Diagnosis, Differential
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Female
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Flow Cytometry
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Gene Expression
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Humans
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Immunophenotyping
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Leukocyte Common Antigens / genetics
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Leukocyte Common Antigens / immunology
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Male
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Middle Aged
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Monoclonal Gammopathy of Undetermined Significance / diagnosis*
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Monoclonal Gammopathy of Undetermined Significance / immunology
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Monoclonal Gammopathy of Undetermined Significance / pathology
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Multiple Myeloma / diagnosis*
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Multiple Myeloma / immunology
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Multiple Myeloma / pathology
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Paraproteinemias / diagnosis*
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Paraproteinemias / immunology
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Paraproteinemias / pathology
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Plasma Cells / immunology
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Plasma Cells / pathology*
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Tetraspanin 28 / genetics
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Tetraspanin 28 / immunology
Substances
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CD56 Antigen
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CD81 protein, human
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NCAM1 protein, human
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Tetraspanin 28
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Leukocyte Common Antigens
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PTPRC protein, human