Sustained donor engraftment in recipients of double-unit cord blood transplantation is possible despite donor-specific human leukoctye antigen antibodies

Biol Blood Marrow Transplant. 2014 May;20(5):735-9. doi: 10.1016/j.bbmt.2014.01.017. Epub 2014 Jan 23.

Abstract

The impact of human leukocyte antigen (HLA) donor-specific antibodies (DSA) on cord blood (CB) engraftment is controversial. We evaluated the influence of pre-existing HLA-antibodies (HLA-Abs) on engraftment in 82 double-unit CB recipients (median age, 48 years) who underwent transplantation for hematologic malignancies. Of 28 patients (34%) with HLA-Abs, 12 had DSA (median mean fluorescence intensity 5255; range, 1057 to 9453). DSA patients had acute leukemia (n = 11) or myelodysplasia (n = 1) and all received either high-dose or reduced-intensity (but myeloablative) conditioning. After myeloablative CB transplantation (CBT) (n = 67), sustained donor engraftment was observed in 95% without HLA-Abs (median, 23 days), 100% with nonspecific HLA-Abs (median, 23 days), and 92% with DSA (median, 31 days, P = .48). Of 6 patients with HLA-Abs to 1 unit, 3 engrafted with that unit and 3 with the other. Of 6 patients with HLA-Abs against both units, 1 had graft failure despite being 100% donor, and 5 engrafted with 1 unit. Successful donor engraftment is possible in patients with DSA after myeloablative double-unit CBT. Our data suggest potential deleterious effects of DSA can be abrogated in patients with hematologic malignancies.

Keywords: Cord blood transplantation; Engraftment; Human leukocyte antigen (HLA) antibodies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antibodies / blood*
  • Child
  • Child, Preschool
  • Cord Blood Stem Cell Transplantation / methods*
  • Female
  • Graft Survival*
  • Graft vs Host Disease / pathology
  • Graft vs Host Disease / prevention & control*
  • HLA Antigens / blood
  • Hematologic Neoplasms / blood
  • Hematologic Neoplasms / mortality
  • Hematologic Neoplasms / pathology
  • Hematologic Neoplasms / therapy*
  • Histocompatibility Testing
  • Humans
  • Male
  • Middle Aged
  • Myeloablative Agonists / therapeutic use*
  • Survival Analysis
  • Transplantation Conditioning*
  • Transplantation, Homologous

Substances

  • Antibodies
  • HLA Antigens
  • Myeloablative Agonists