Abstract
Here we report a spectroscopic, electrochemical and computational study of cytochrome c showing that nitration of Tyr74 induces Tyr deprotonation, which is coupled to Met/Lys axial ligand exchange, and results in concomitant gain of peroxidatic activity at physiological pH.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Computer Simulation
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Cytochromes c / chemistry*
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Hydrogen-Ion Concentration
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Ligands
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Models, Molecular
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Nitrates / chemistry*
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Oxidation-Reduction
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Protons*
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Thermodynamics
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Tyrosine / chemistry*
Substances
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Ligands
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Nitrates
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Protons
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Tyrosine
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Cytochromes c