Cryopreservation with dimethyl sulfoxide prevents accurate analysis of skinned skeletal muscle fibers mitochondrial respiration

Biochimie. 2014 May:100:227-33. doi: 10.1016/j.biochi.2014.01.014. Epub 2014 Jan 25.

Abstract

Impact of cryopreservation protocols on skeletal muscle mitochondrial respiration remains controversial. We showed that oxygen consumption with main mitochondrial substrates in rat skeletal muscles was higher in fresh samples than in cryopreserved samples and that this difference was not fixed but grow significantly with respiration rates with wide fluctuations around the mean difference. Very close results were observed whatever the muscle type and the substrate used. Importantly, the deleterious effects of ischemia-reperfusion observed on fresh samples vanished when cryopreserved samples were studied. These data demonstrate that this technic should probably be performed only extemporaneously.

Keywords: Cryopreservation; DMSO; Ischemia; Mitochondria; Muscle; Oxygraphy.

MeSH terms

  • Animals
  • Artifacts*
  • Cell Respiration / drug effects
  • Cryopreservation*
  • Cryoprotective Agents / pharmacology*
  • Dimethyl Sulfoxide / pharmacology
  • Male
  • Mitochondria / drug effects*
  • Mitochondria / metabolism
  • Muscle Fibers, Skeletal / drug effects*
  • Muscle Fibers, Skeletal / metabolism
  • Oxidative Phosphorylation / drug effects
  • Rats
  • Rats, Wistar

Substances

  • Cryoprotective Agents
  • Dimethyl Sulfoxide