Evaluation of a commercial real-time PCR for the detection of extended spectrum β-lactamase genes

Ina Willemsen; Linda Hille; Angela Vrolijk; Anneke Bergmans; Jan Kluytmans

doi:10.1099/jmm.0.070110-0

Evaluation of a commercial real-time PCR for the detection of extended spectrum β-lactamase genes

J Med Microbiol. 2014 Apr;63(Pt 4):540-543. doi: 10.1099/jmm.0.070110-0. Epub 2014 Jan 28.

Authors

Ina Willemsen¹, Linda Hille¹, Angela Vrolijk¹, Anneke Bergmans^{2

1}, Jan Kluytmans^{3

2

1}

Affiliations

¹ Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
² Department for Microbiology and Infection Control, Saint Fransiscus Hospital, Roosendaal, The Netherlands.
³ Department for Microbiology and Infection Control, VUmc Medical Center, Amsterdam, The Netherlands.

PMID: 24473202
DOI: 10.1099/jmm.0.070110-0

Abstract

We investigated the performance of a real-time PCR for the detection of extended spectrum β-lactamase genes in Enterobacteriaceae (Check-MDR ESBL PCR). Results from micro-arrays were considered as the gold standard. An analysis on 489 isolates resulted in a sensitivity of 98.9 % and a specificity of 100 % for the PCR.

Publication types

Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Enterobacteriaceae / enzymology*
Enterobacteriaceae / genetics*
Enterobacteriaceae / isolation & purification
Enterobacteriaceae Infections / microbiology
Humans
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
beta-Lactamases / genetics*

Substances

beta-Lactamases