The genetic incorporation of thirteen novel non-canonical amino acids

Alfred Tuley; Yane-Shih Wang; Xinqiang Fang; Yadagiri Kurra; Yohannes H Rezenom; Wenshe R Liu

doi:10.1039/c3cc49068h

The genetic incorporation of thirteen novel non-canonical amino acids

Chem Commun (Camb). 2014 Mar 11;50(20):2673-5. doi: 10.1039/c3cc49068h.

Authors

Alfred Tuley¹, Yane-Shih Wang, Xinqiang Fang, Yadagiri Kurra, Yohannes H Rezenom, Wenshe R Liu

Affiliation

¹ Department of Chemistry, Texas A&M University, College Station, TX 77843, USA. [email protected].

Abstract

Thirteen novel non-canonical amino acids were synthesized and tested for suppression of an amber codon using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair. Suppression was observed with varied efficiencies. One non-canonical amino acid in particular contains an azide that can be applied for site-selective protein labeling.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acids / genetics*
Codon, Terminator
Molecular Structure
Phenylalanine / genetics*

Substances

Amino Acids
Codon, Terminator
Phenylalanine

Abstract

Publication types

MeSH terms

Substances

Grants and funding