Abstract
Nine cembranoid diterpenes 1-9, including four new compounds, crassumols D-G (1-4), were isolated from the methanol extract of the Vietnamese soft coral Lobophytum crassum. Spectroscopic methods were used to elucidate the structures of these compounds. Compound 5 exhibited a potent inhibitory effect on tumor necrosis factor-alpha (TNFα)-induced nuclear factor-kappa B (NF-κB) transcriptional activation in HepG2 cells and significantly inhibited the mRNA expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in a dose-dependent manner.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anthozoa / chemistry*
-
Anti-Inflammatory Agents / chemistry*
-
Anti-Inflammatory Agents / isolation & purification
-
Anti-Inflammatory Agents / pharmacology*
-
Cyclooxygenase 2 / genetics
-
Diterpenes / chemistry*
-
Diterpenes / isolation & purification
-
Diterpenes / pharmacology*
-
Hep G2 Cells
-
Humans
-
NF-kappa B / genetics
-
Nitric Oxide Synthase Type II / genetics
-
RNA, Messenger / genetics
-
Transcriptional Activation / drug effects
-
Tumor Necrosis Factor-alpha / immunology*
Substances
-
Anti-Inflammatory Agents
-
Diterpenes
-
NF-kappa B
-
RNA, Messenger
-
Tumor Necrosis Factor-alpha
-
Nitric Oxide Synthase Type II
-
Cyclooxygenase 2