Preparation, characterization and cytotoxicity of schizophyllan/silver nanoparticle composite

A M Abdel-Mohsen; Rasha M Abdel-Rahman; Moustafa M G Fouda; L Vojtova; L Uhrova; A F Hassan; Salem S Al-Deyab; Ibrahim E El-Shamy; J Jancar

doi:10.1016/j.carbpol.2013.11.040

Preparation, characterization and cytotoxicity of schizophyllan/silver nanoparticle composite

Carbohydr Polym. 2014 Feb 15:102:238-45. doi: 10.1016/j.carbpol.2013.11.040. Epub 2013 Dec 4.

Authors

A M Abdel-Mohsen¹, Rasha M Abdel-Rahman², Moustafa M G Fouda³, L Vojtova⁴, L Uhrova⁵, A F Hassan⁶, Salem S Al-Deyab⁷, Ibrahim E El-Shamy⁸, J Jancar⁹

Affiliations

¹ Central European Institute of Technology (CEITEC), Brno University of Technology, Brno Czech Republic; Textile Research Division, National Research Center, Dokki, P.O. 12622, Giza 12522, Egypt. Electronic address: [email protected].
² Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Czech Republic.
³ Chemistry Department, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia; Textile Research Division, National Research Center, Dokki, P.O. 12622, Giza 12522, Egypt. Electronic address: [email protected].
⁴ Central European Institute of Technology (CEITEC), Brno University of Technology, Brno Czech Republic.
⁵ Institute of Materials Chemistry, Brno University of Technology, Czech Republic.
⁶ Chemistry Department, Faculty of Science, Damanhour University, Damanhour, Egypt.
⁷ Chemistry Department, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
⁸ Chemistry Department, Faculty of Science, Fayoum University, Fayoum, Egypt.
⁹ Central European Institute of Technology (CEITEC), Brno University of Technology, Brno Czech Republic; Institute of Materials Chemistry, Brno University of Technology, Czech Republic.

PMID: 24507278
DOI: 10.1016/j.carbpol.2013.11.040

Abstract

Silver nanoparticles (Ag-NPs) have been successfully prepared with a simple and "green" chemical reduction method. Triple helical schizophyllan (SPG) was used for the first time as reducing and stabilizing agents. The effect of temperature, silver nitrate/schizophyllan concentrations, pH of the reactions medium and the reaction time were investigated. The obtained schizophyllan/Ag-NP was characterized by UV-vis spectroscopy, TEM, DLS, X-ray diffraction, TGA, and ATR-FTIR. The results revealed that, Ag-NPs attached to SPG through a strong non-covalent interaction, leading to good dispersion of Ag-NPs with a diameter of 6 nm within the biopolymer matrix. By increasing the pH of the reaction medium, the triple helical structure of SPG was partially broken. The SPG/AgNP nanocomposite was non-toxic for mouse fibroblast line (NIH-3T3) and human keratinocyte cell line (HaCaT).

Keywords: Cytotoxicity; Green synthesis; Schizophyllan; Silver nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Humans
Hydrogen-Ion Concentration
Keratinocytes / drug effects
Metal Nanoparticles / chemistry
Metal Nanoparticles / toxicity*
Mice
Microscopy, Electron, Transmission
NIH 3T3 Cells
Silver / chemistry*
Sizofiran / chemistry
Sizofiran / toxicity*
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Temperature
X-Ray Diffraction

Substances

Silver
Sizofiran