Genetic manipulation of Streptococcus pyogenes (the Group A Streptococcus, GAS)

Yoann Le Breton; Kevin S McIver

doi:10.1002/9780471729259.mc09d03s30

Genetic manipulation of Streptococcus pyogenes (the Group A Streptococcus, GAS)

Curr Protoc Microbiol. 2013 Oct 2:30:9D.3.1-9D.3.29. doi: 10.1002/9780471729259.mc09d03s30.

Authors

Yoann Le Breton¹, Kevin S McIver¹

Affiliation

¹ Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland.

Abstract

Streptococcus pyogenes (the Group A Streptococcus, GAS) is a Gram-positive bacterium responsible for a wide spectrum of diseases ranging from mild superficial infections (pharyngitis, impetigo) to severe, often life-threatening invasive diseases (necrotizing fasciitis, streptococcal toxic shock syndrome) in humans. This unit describes molecular techniques for the genetic manipulation of S. pyogenes with detailed protocols for transformation, gene disruption, allelic exchange, transposon mutagenesis, and genetic complementation.

Keywords: Streptococcus pyogenes; genetic complementation; genomic DNA; mariner; plasmid; transformation; transposon mutagenesis.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Gene Deletion
Genetic Complementation Test
Genetics, Microbial / methods*
Molecular Biology / methods*
Mutagenesis, Insertional
Streptococcus pyogenes / genetics*
Transformation, Bacterial

Abstract

Publication types

MeSH terms

Grants and funding