Intra-molecular reactions as a new approach to investigate bio-radical reactivity: a case study of cysteine sulfinyl radicals

Analyst. 2014 Mar 21;139(6):1327-30. doi: 10.1039/c3an02307a.

Abstract

Herein, we demonstrated the use of gas-phase intra-molecular reactions facilitated by collisional activation to investigate bi-molecular reactions with inherent low reactivity. Reactions between sulfinyl radicals (-SO˙) toward free thiol (-SH) were employed as a model system. A new reaction channel, i.e. sulfinyl exchange with thiol, was observed under beam-type collision-induced dissociation (CID), which was not detectable from traditional ion/molecule reactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cysteine / chemistry*
  • Free Radicals / chemistry*
  • Gases / chemistry
  • Ions / chemistry
  • Mass Spectrometry
  • Peptides / chemistry*
  • Sulfhydryl Compounds / chemistry*

Substances

  • Free Radicals
  • Gases
  • Ions
  • Peptides
  • Sulfhydryl Compounds
  • Cysteine