Hsa-miR-125b suppresses bladder cancer development by down-regulating oncogene SIRT7 and oncogenic long noncoding RNA MALAT1

Yonghua Han; Yuchen Liu; Hu Zhang; Tiantian Wang; Ruiying Diao; Zhimao Jiang; Yaoting Gui; Zhiming Cai

doi:10.1016/j.febslet.2013.10.023

Hsa-miR-125b suppresses bladder cancer development by down-regulating oncogene SIRT7 and oncogenic long noncoding RNA MALAT1

FEBS Lett. 2013 Oct 25:S0014-5793(13)00780-1. doi: 10.1016/j.febslet.2013.10.023. Online ahead of print.

Authors

Yonghua Han¹, Yuchen Liu¹, Hu Zhang², Tiantian Wang², Ruiying Diao¹, Zhimao Jiang², Yaoting Gui³, Zhiming Cai⁴

Affiliations

¹ Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, Postdoctroal Scientific Research Base, Zhongshan School of Medicine, Sun Yat-sen University, Shenzhen, China.
² Guangdong Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, China.
³ Guangdong Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, China. Electronic address: [email protected].
⁴ Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, Postdoctroal Scientific Research Base, Zhongshan School of Medicine, Sun Yat-sen University, Shenzhen, China. Electronic address: [email protected].

PMID: 24512851
DOI: 10.1016/j.febslet.2013.10.023

Abstract

MicroRNAs mainly inhibit coding genes and long non-coding RNA expression. Here, we report that hsa-miR-125b and oncogene SIRT7/ oncogenic long noncoding RNA MALAT1 were inversely expressed in bladder cancer. Hsa-miR-125b mimic downregulated, whereas hsa-miR-125b inhibitor upregulated the expression of SIRT7 and MALAT1. Binding sites were confirmed between hsa-miR-125b and SIRT7/MALAT1. Upregulation of hsa-miR-125b or downregulation of SIRT7 inhibited proliferation, motility and increased apoptosis. The effects of upregulation of hsa-miR-125b were similar to that of silencing MALAT1 in bladder cancer as we had previously described. These data suggest that hsa-miR-125b suppresses bladder cancer development via inhibiting SIRT7 and MALAT1.

Keywords: Bladder cancer; Hsa-miR-125b; Long noncoding RNA; MALAT1; Microrna; SIRT7.