First isolation and molecular characterization of Toxoplasma gondii from a human placenta in Argentina

Lais Pardini; Liliana A Carral; Mariana Bernstein; María L Gos; Patricia Olejnik; Juan M Unzaga; Federico J Kaufer; Cristina B Freuler; Ricardo A Durlach; María C Venturini

doi:10.1016/j.parint.2013.10.011

First isolation and molecular characterization of Toxoplasma gondii from a human placenta in Argentina

Parasitol Int. 2014 Apr;63(2):470-2. doi: 10.1016/j.parint.2013.10.011. Epub 2013 Oct 26.

Affiliations

¹ Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina; CONICET, Argentina. Electronic address: [email protected].
² Centro de toxoplasmosis y otras zoonosis, Hospital Alemán, Buenos Aires, Argentina.
³ Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina.
⁴ Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina; CONICET, Argentina.

PMID: 24513795
DOI: 10.1016/j.parint.2013.10.011

Abstract

Blood sample and placenta were taken from a 37-week pregnant woman; serologic results indicated acute toxoplasmosis. Placenta was inoculated into mice. Seropositive mice were sacrificed and tissue cysts from brain were inoculated into new mice. Specific DNA was detected by PCR, and the isolate was characterized as Type II by nPCR-RFLP for nSAG2, SAG3, BTUB, GRA6, c29-2, c22-8, L358, PK1 and Apico markers. This is the first isolation and molecular characterization of Toxoplasma gondii from humans in Argentina.

Keywords: Genotyping; Human; Isolation; Placenta; Toxoplasma gondii.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Argentina / epidemiology
Biological Assay
Coccidiostats / therapeutic use
Female
Humans
Infectious Disease Transmission, Vertical*
Mice
Placenta / parasitology*
Pregnancy
Spiramycin / therapeutic use
Toxoplasma / isolation & purification*
Toxoplasmosis / drug therapy
Toxoplasmosis / epidemiology*
Toxoplasmosis / transmission*

Substances

Coccidiostats
Spiramycin