A novel hapten and monoclonal-based enzyme-linked immunosorbent assay for sulfonamides in edible animal tissues

Food Chem. 2014 Jul 1:154:52-62. doi: 10.1016/j.foodchem.2014.01.016. Epub 2014 Jan 14.

Abstract

For high-throughput monitoring of the residues of sulfonamides (SAs) in edible animal tissues, a novel hapten and monoclonal-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed. The novel hapten was synthesized and conjugated to carrier protein as immunogen. The spleen cells of the inoculated mice expressing group-specificity against SAs were fused. The obtained monoclonal antibody 4E5 showed the cross-reactivity (CR) to 16 structurally different SAs. Based on this antibody, an optimised icELISA protocol was carried out with only phosphate-buffered saline for the fast extraction of SAs in the tissues. The limits of detection of SAs in chicken ranged from 1.5 to 22.3μgkg(-1). The recoveries were 70.6-121% with less than 24.1% relative standard deviation. The developed ic-ELISA showed a good correlation with high performance liquid chromatography. It would be a useful tool for the screening of residues of SAs in edible animal tissues.

Keywords: Edible animal tissues; Hapten; Indirect competitive enzyme-linked immunosorbent assay; Monoclonal antibody; Sulfonamides.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / analysis*
  • Chickens
  • Drug Residues / analysis*
  • Enzyme-Linked Immunosorbent Assay / instrumentation
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Food Contamination / analysis*
  • Haptens / chemistry
  • Meat / analysis*
  • Mice
  • Mice, Inbred BALB C
  • Muscle, Skeletal / chemistry*
  • Sulfonamides / analysis*

Substances

  • Anti-Bacterial Agents
  • Haptens
  • Sulfonamides