IL-27 improves migrational and antiviral potential of CB dendritic cells

Julia Birkholz; Aysefa Doganci; Claudia Darstein; Stephan Gehring; Fred Zepp; Claudius U Meyer

doi:10.1016/j.humimm.2014.02.004

IL-27 improves migrational and antiviral potential of CB dendritic cells

Hum Immunol. 2014 Jun;75(6):584-91. doi: 10.1016/j.humimm.2014.02.004. Epub 2014 Feb 12.

Authors

Julia Birkholz¹, Aysefa Doganci², Claudia Darstein², Stephan Gehring², Fred Zepp², Claudius U Meyer²

Affiliations

¹ Pediatric Immunology Mainz, Children's Hospital, Medical Center of the Johannes Gutenberg University Mainz, Germany. Electronic address: [email protected].
² Pediatric Immunology Mainz, Children's Hospital, Medical Center of the Johannes Gutenberg University Mainz, Germany.

PMID: 24530744
DOI: 10.1016/j.humimm.2014.02.004

Abstract

Interleukin (IL)-27 is known to be increased considerably in cord blood (CB) dendritic cells (DCs) after TLR ligation. Previously, we demonstrated that also basal IL-27 levels are higher in CB DCs. Here, we examined effects of IL-27 on monocyte derived dendritic cells (moDCs) to approach its particular role in the specialized immune system of the human neonate. Exogenous IL-27 promotes IL-27 transcription in CB and adult blood (AB) moDCs. IL-27 acts on CB moDCs primarily by significantly augmenting IL-27 protein, secondarily by increasing transcription of CXCL10 among other chemokines, chemokine receptor CCR1, interferon stimulated genes, transcription factor IRF8 and genes involved in antigen presentation. Furthermore, CB moDCs respond to IL-27 with augmented IL-8 and Tumor necrosis factor (TNF)-α. The results suggest that IL-27 enhances migrational and antiviral properties of CB dendritic cells.

MeSH terms

Adult
Cell Differentiation
Cell Movement / drug effects*
Cells, Cultured
Chemokine CXCL10 / genetics
Chemokine CXCL10 / metabolism
Dendritic Cells / cytology
Dendritic Cells / drug effects
Dendritic Cells / metabolism*
Fetal Blood / cytology*
Fetal Blood / metabolism
Gene Expression Regulation
Humans
Infant, Newborn
Interferon Regulatory Factors / genetics
Interferon Regulatory Factors / metabolism
Interleukin-8 / genetics
Interleukin-8 / metabolism
Interleukins / biosynthesis
Interleukins / genetics*
Interleukins / pharmacology
Monocytes / cytology
Monocytes / metabolism
Receptors, CCR1 / genetics
Receptors, CCR1 / metabolism
STAT1 Transcription Factor / genetics
STAT1 Transcription Factor / metabolism
Signal Transduction
Transcription, Genetic
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / metabolism

Substances

CCR1 protein, human
CXCL10 protein, human
Chemokine CXCL10
Interferon Regulatory Factors
Interleukin-8
Interleukins
MYDGF protein, human
Receptors, CCR1
STAT1 Transcription Factor
STAT1 protein, human
Tumor Necrosis Factor-alpha
interferon regulatory factor-8