Magnetic ligation method for quantitative detection of microRNAs

Adv Healthc Mater. 2014 Jul;3(7):1015-9. doi: 10.1002/adhm.201300672. Epub 2014 Feb 14.

Abstract

A magnetic ligation method is utilized for the detection of microRNAs among a complex biological background without polymerase chain reaction or nucleotide modification. The sandwich probes assay can be adapted to analyze a panel of microRNAs associated with cardiovascular diseases in heart tissue samples.

Keywords: diagnostics; enzymatic ligation; magnetic labeling; magnetic resonance; microRNA.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA Ligases
  • Magnetite Nanoparticles / chemistry*
  • MicroRNAs / analysis*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Microspheres
  • Molecular Probe Techniques*
  • Nucleic Acid Amplification Techniques / methods*

Substances

  • Magnetite Nanoparticles
  • MicroRNAs
  • DNA Ligases