Nucleotides as nontoxic endogenous endosomolytic agents in drug delivery

Hana Cho; Yong-Yeon Cho; You Han Bae; Han Chang Kang

doi:10.1002/adhm.201400008

Nucleotides as nontoxic endogenous endosomolytic agents in drug delivery

Adv Healthc Mater. 2014 Jul;3(7):1007-14. doi: 10.1002/adhm.201400008. Epub 2014 Feb 17.

Authors

Hana Cho¹, Yong-Yeon Cho, You Han Bae, Han Chang Kang

Affiliation

¹ Department of Pharmacy and Integrated Research, Institute of Pharmaceutical Sciences, College of Pharmacy, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do, 420-743, Republic of Korea.

PMID: 24535942
DOI: 10.1002/adhm.201400008

Abstract

Nontoxic endogenous nucleotides such as adenosine triphosphate and guanosine triphosphate have secondary phosphate groups, causing proton-buffering capacity and/or hemolytic activity in endolysosomal pH ranges. Nucleotides co-delivered in single polymeric pDNA nanocarrier induce highly enhanced transfection efficiency with negligible cytotoxicity due to their endosomolytic functions.

Keywords: ATP; drug delivery; endosomolytic material; gene delivery; nucleotide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Survival / drug effects
Drug Delivery Systems / methods*
HEK293 Cells
HeLa Cells
Humans
Intracellular Space / metabolism
Nanostructures / chemistry*
Nanostructures / toxicity*
Nucleotides / chemistry*
Nucleotides / toxicity*
Transfection

Substances

Nucleotides