Diagnosis of oral mucous membrane pemphigoid by means of combined serologic testing

Taihei Hayakawa; Minao Furumura; Hideo Fukano; Xiaoguang Li; Norito Ishii; Takahiro Hamada; Chika Ohata; Daisuke Tsuruta; Kazuo Shimozato; Takashi Hashimoto

doi:10.1016/j.oooo.2013.12.402

Diagnosis of oral mucous membrane pemphigoid by means of combined serologic testing

Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Apr;117(4):483-96. doi: 10.1016/j.oooo.2013.12.402. Epub 2013 Dec 20.

Authors

Affiliations

¹ Postgraduate Researcher, Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Japan; Postgraduate Student, Department of Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, Japan.
² Associate Professor, Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Japan.
³ Associate Professor, Department of Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, Japan.
⁴ Postdoctoral Researcher, Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Japan.
⁵ Senior Lecturer, Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Japan.
⁶ Professor, Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
⁷ Professor, Department of Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, Japan.
⁸ Professor, Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, Kurume, Japan. Electronic address: [email protected].

PMID: 24556492
DOI: 10.1016/j.oooo.2013.12.402

Abstract

Objective: Mucous membrane pemphigoid (MMP) is a rare autoimmune bullous disease caused by various autoantibodies. This study aimed to evaluate the diagnostic value of MMP-specific autoantibodies in patient sera.

Study design: We analyzed sera from 30 MMP-suspected patients with intractable oral mucosal lesions using a combination of indirect immunofluorescence with 1M NaCl-split skin, immunoblot analysis, and ELISAs. We also analyzed clinical features among different types of MMP.

Results: Seventeen, 4, and 3 patients were diagnosed with anti-BP180-type MMP, anti-laminin-332-type MMP, and combined anti-BP180/anti-laminin-332-type MMP, respectively.

Conclusions: Our results indicated that a combination of immunologic testing for circulating autoantibodies is useful for the diagnosis of MMP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Aged, 80 and over
Diagnosis, Differential
Female
Humans
Male
Middle Aged
Mouth Diseases / blood*
Mouth Diseases / immunology
Pemphigoid, Benign Mucous Membrane / blood*
Pemphigoid, Benign Mucous Membrane / immunology
Serologic Tests / methods*